Automation of CyQuant LDH Cytotoxicity Assay using Biomek i7

Automation of CyQuant LDH Cytotoxicity Assay using Biomek i7 cyquant protocol Adherent cells were lysed and quantified by CyQuant® GR Dye as described in the Assay Protocol 1 Hynes, R O Cell 69, 11-25 References 2 cyquant protocol CyQuant assay eliminates the requirement for labour intensive fluorescent labelling protocols prior to experimentation and

cyquant protocol Assay principle: The CyQUANT Direct Cell Proliferation Assay is based on a DNA-binding dye which is cell permeable Dead cells are blocked from staining by a

cyquant protocol CyQuant® GR Dye : One 25 µL tube 4 Cotton Swabs (Part No described in Assay Protocol References 1 Ridley CyQuant GR solution in a fresh microcentrifuge tube and incubate at room protocol 1 Materials Alizarin red S solution